This course offers no-nonsense, results-oriented training that will help you improve your bottom-line performance. Come prepared to discuss your analytical problems and challenges with expert chromatographers. By enrolling in this course, you will learn to master state-of-the-art techniques and equipment in HPLC with practical, hands-on experience. In the course's hands-on laboratory sessions, you will work on a variety of experiments, and in the process, become familiar with HPLC hardware; column dimensions; mobile phase strength, flow rate, and temperature; and internal vs. external HPLC is a technique for separation, identification and quantification of components in a mixture. It is especially suitable for compounds that are not easily volatilized, thermally unstable, and have high molecular weights. The liquid phase is pumped at a constant rate to the column packed with the stationary phase. Before entering the column, the analysis sample is injected into the carrier stream. On reaching the column the sample components are selectively retained on the basis of physic-chemical interactions between the analytic molecules and the stationary phase. The mobile phase moving at a steady rate elutes the components based on the operating conditions. Detection techniques are employed for the detection and quantification of the eluted components. High-performance liquid chromatography is basically a highly improved form of column chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. That makes it much faster. It also allows you to use a very much smaller particle size for the column packing material which gives a much greater surface area for interactions between the stationary phase and the molecules flowing past it. This allows a much better separation of the components of the mixture. The other major improvement over column chromatography concerns the detection methods which can be used. These methods are highly automated and extremely sensitive. Standard. By the end of the course, you will know how to develop a method for a complete unknown mixture.
By the end of this course delegates will be able to:
DAY 1
HPLC Overview
Practical and Theoretical Principles
HPLC Instrumentation
Performing an Analysis
DAY 2
Solvent Preparation, Isocratic, and Gradient-Elution
High-Performance Liquid Chromatography Overview
DAY 3
Separation Fundamentals and Mobile Phase
Separation Modes and Columns
Day 4
Gradients, Method Development, and Gradient Elution
DAY 5
Quantitative Analysis and Troubleshooting
This course is intended for Laboratory Personnel and Technical Staff such as Chemists, Analysts, Chemical Engineers, and anybody interested in chemical analysis, research and development, environmental studies, quality control, refineries, petrochemical plants, water, and wastewater plants, hospitals, and medical centers. Key Features of This Dynamic Course