The course will provide exposure to the advances in the field of Atomic Absorption Spectroscopy to the experienced analyst and both basics and practical aspects to beginners. Wide coverage is given to fundamental concepts of laboratory operations in a lucid manner so that both the novice and expert will find the programmer as a useful reference for the day to day laboratory operations. Atomic Absorption spectroscopy is extensively used in analysis of trace metals in PPM to sub PPM levels using absorption of element specific wavelengths by atoms in ground state. It has found applications in water analysis, foods & beverages, agricultural products, geological samples and body fluids.
Upon the successful completion of this course, each participant will be able to:-
- R&D Scientists
- QA Managers & Practicing Chemists
- Students in disciplines of Chemistry, Pharmacy, Food & Nutrition and Agricultural Sciences.
- Geological and Mining scientists
- Clinical researchers
- Governmental Regulatory Bodies
- Environmental Scientists
The course will provide exposure to the advances in the field of Atomic Absorption Spectroscopy to the experienced analyst and both basics and practical aspects to beginners. Wide coverage is given to fundamental concepts of laboratory operations in a lucid manner so that both the novice and expert will find the programmer as a useful reference for the day to day laboratory operations
Day 1
Introduction
Spectroscopic Analysis
- Historical and review
- Basic Principals
- Electromagnetic Spectrum
- Beer–Lambert Law
- Theory of Spectroscopy
- Absorption
- Emission
- Fluorescence
- Phosphorescence
- Type of Spectroscopy Analysis
- Molecular Spectroscopy analysis
- Atomic Spectroscopy analysis
Day 2
Atomic Absorption Spectroscopy (AAS)
AAS Instrumentation & components:
- Atomization
- Flame AAS
- Graphite Furnace AAS
- Effect of Temperature on Absorption
- Radiation Sources
- Hollow-Cathode Lamps (HCL)
- Electrodeless Discharge Lamps (EDL)
- Optical System
- Single beam
- Double beam
- Monochrometor
Day 3
AAS Instrumentation:
- Detector
- Photomultiplier tube (PMT)
- Charged Coupled Device (CCD)
- Computer interface
- Comparing & Evolution Spectroscopic Techniques
- AAS; Advantages and Disadvantages
- Detection Limits
- Limit of detection (LOD)
- Limit of quantitative measurement (LOQ)
- Limit of Linearity (LOL)
Day 4
AAS; Maintenance & Troubleshooting
- Interferences
- Spectral Interference
- Chemical Interference
- Ionization Interference
- Matrix Interference
- Background Correction
- Duterium lamp correction
- Zeeman correction
- Types of calibration methods
- Standard calibration
- Standard addition calibration
- Internal standard calibration
Day 5
AAS; Maintenance & Troubleshooting
- Routine Maintenance
- Problem and troubleshot
- Safety requirement for running
AAS; Analysis and Application:
- Sampling , Preparation and Treatment
- Organic & Inorganic sample analysis
- Cold vapor technique
- Petroleum Analysis
- Water Analysis
- Solid Waste Analysis
- Environmental Analysis
- Result data analysis & evolution
All Lab Technical Staff & Managers.